Habits of mind are important things to be equipped to prospective teachers. In order to be able to solve every problem they face, prospective teachers should be used to have self-regulation, critical thinking, and creative thinking. Therefore, to achieve these objectives, appropriate learning experience and strategies are needed. In this article, an entrepreneurship-oriented 5E (Engagement, Explain, Exploration, Elaboration, and Evaluation) inquiry learning program is developed, it is also called 5E+e. The focus of this article is to investigate the improvement of prospective teachers’ Habits of Mind during horticulture course through the 5E+e inquiry learning program. The quasy experiment method was used in this study with one group pretest-posttest design with the involvement of 31 Biology Education students in one teachers Institution in Central Java on their sixth semester who took the horticulture course. Data were collected using questionnaires and observation sheets in the form of a Habits of Mind rubric adapted from the Marzano’s framework. Data were then analyzed in quantitative and in qualitative descriptive manner from various sources, so as to obtain comprehensive conclusions. Research results show an improvement on the average Habits of Mind score of prospective teachers, where the average score collected before the program was given was at 2.68; and the average score after being given the program was at 3.34 of 4.00 scale. The calculated N-gain is 0.31; which is in the moderate category. The data were supported by the results of observations during the learning process which at each stage shows progress, i.e. stage I (3.11), stage II (3.08), stage III (3.39), and stage IV (3.59). Based on the findings, students can cultivate horticultural crops creatively with a variety of planting techniques, critical when observing plant growth and development, and diligent in maintaining a successful crop cultivation project. Thus, it can be concluded that 5E+e inquiry learning program implemented in horticultural course can greatly improve the Habits of Mind of prospective teachers.

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