The aim of this study is to determine  the learning styles of the students of the schools that accept students by entrance exam and their attitudes towards biology course. This study was carried out by using school survey which is one of the descriptive methods. Learning style inventory and biology course scale were applied to 266 ninth-grade students of three different schools. 1. %19,82 of the students of the Anatolian High School is imaginative, %43,96 is analytical, %29,31 is common sense and %6,89 is dynamicaly learning; %13,75 of the students of the Anatolian Teachers’ High School is imaginative, %51,25 is analytical, %30 is common sense and %5 is dynamicaly learning; %14,28 of the students of the Science High School is imaginative, %47,14 is analytical, %28,57 is common sense and %10 is dynamicaly learning. 2. A significant attitude difference towards biology course (p< ,05) was detected in terms of gender. The attitude of the girls is more positive. 3. According to school variable, there is a major difference among attitudes of the students towards biology course.There is an important attitude score difference between the Anatolian High School and the Science High School and between the Anatolian Teachers’ High School and the Science High Schools.(p< ,001) The scores of the Anatolian High School  and the Anatolian Teachers’ High  School are better.  4. According to learning styles, there is an important attitude score difference between the Second Type Learners and Third Type Learners (p< ,05). The scores are in favor of the Third Type Learners.